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Odling och manipulering av O9-1 Neural Crest celler
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Odling och manipulering av O9-1 Neural Crest celler

Article DOI: 10.3791/58346-v • 08:32 min • October 9th, 2018
October 9th, 2018 •
1, 2, 2, 1
1Molecular Physiology and Biophysics, Baylor College of Medicine, 2Department of Biochemistry and Molecular Biology, University of Southern California

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O9-1 är en multipotenta mus neural crest cellinje. Här beskriver vi detaljerade stegvisa protokoll för odling O9-1 celler, differentiera O9-1 celler till specifika celltyper och genetiskt manipulera O9-1 celler med siRNA-medierad knockdown eller CRISPR-Cas9 gen editering.

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Genetik utfärda 140 CRISPR Cas9 O9-1 celler gen knockout gen knockdown neural crest celler
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