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JoVE Journal Immunology and Infection
Misurazione di High-throughput di membrana plasmatica risigillazione efficienza in cellule di mammifero
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Misurazione di High-throughput di membrana plasmatica risigillazione efficienza in cellule di mammifero

Article DOI: 10.3791/58351-v • 10:07 min • January 7th, 2019
January 7th, 2019 •
1,2,3, 4, 1,2,3
1Department of Microbial Infection and Immunity, The Ohio State University, 2Department of Microbiology, The Ohio State University, 3Infectious Diseases Institute, The Ohio State University, 4Division of Biostatistics, College of Public Health, The Ohio State University

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Qui descriviamo un'analisi fluorescenza-basata di alto-rendimento che misura la membrana plasmatica risigillazione efficienza attraverso analisi fluorometriche e imaging in cellule viventi. Questo test può essere utilizzato per lo screening di farmaci o geni che regolano la chiusura della membrana plasmatica in cellule di mammifero.

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Immunologia e infezione numero 143 membrana plasmatica una chiusura riparazione della membrana distrofia muscolare ioduro di propidio acido nucleico carbocyanine associazione tintura pore-forming tossina Listeriolisina O
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