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JoVE Journal Developmental Biology
Visualiser les Drosophila jambe axones des motoneurones à travers la cuticule de l’adulte
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Visualiser les Drosophila jambe axones des motoneurones à travers la cuticule de l’adulte

Article DOI: 10.3791/58365-v • 08:33 min • October 30th, 2018
October 30th, 2018 •
1, 2, 3, 2, 1
1Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon, CNRS, 2Departments of Biochemistry and Molecular Biophysics, and Neuroscience, Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, 3Neuroscience Program, NYU School of Medicine

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Nous décrivons ici un protocole afin de visualiser le ciblage axonale avec une protéine fluorescente dans jambes adultes de la drosophile par fixation, montage, imagerie et imageries après étapes.

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Numéro 140 drosophile les motoneurones jambe microscopie confocale biologie du développement axones imagerie protéine fluorescente verte (GFP) cuticule
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