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Journal / Immunology and Infection / Oral Intubation of Adult Zebrafish: A Model for…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Oral Intubation of Adult Zebrafish
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Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds

Article DOI: 10.3791/58366-v • 04:33 min • September 27th, 2018
September 27th, 2018 •
1,2, 1,2, 1,2
1Institute of Biotechnology and Biomedicine (IBB-UAB), Universitat Autònoma de Barcelona, 2Department of Cell Biology, Animal Physiology and Immunology, Universitat Autònoma de Barcelona

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Summary
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The protocol describes intubating adult zebrafish with a biologic; then dissecting and preparing the intestine for cytometry, confocal microscopy and qPCR. This method allows administration of bioactive compounds to monitor intestinal uptake and the local immune stimulus evoked. It is relevant for testing the intestinal dynamics of oral prophylactics.

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Oral Intubation Adult Zebrafish Intestinal Uptake Bioactive Compounds Mucosa Vaccinology Immune System Modulation Simple Setup Low Mortality Rates Insight Into Mucosal Vaccinology Application To Other Compounds Visual Demonstration Silicone Tubing Luer-lock Needle Filter Pipette Tip 100-microliter Luer-lock Syringe Experimental Tanks Nanoparticle Solution Anesthetizing Fish
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