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Journal / Chemistry / Sentez ve μ-Conotoxin PIIIA izomerler farklı…
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Chemistry

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JoVE Journal Chemistry
Sentez ve μ-Conotoxin PIIIA izomerler farklı disülfür Connectivities ile yapı belirlenmesi
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Sentez ve μ-Conotoxin PIIIA izomerler farklı disülfür Connectivities ile yapı belirlenmesi

Article DOI: 10.3791/58368-v • 11:44 min • October 2nd, 2018
October 2nd, 2018 •
*1, *1, *1, 1
1Pharmaceutical Biochemistry and Bioanalytics, Pharmaceutical Institute, University of Bonn
* These authors contributed equally

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Sistein-zengin peptidler onların disülfür bağlantısı bağlı olarak farklı üç boyutlu yapılar içine katlayın. Arabellek oksidasyon istenen disülfür bağlantı yol değil tek tek disülfit izomerler hedeflenen sentezi gereklidir. Protokol ile 3 disülfür bağ peptidler ve NMR ve MS/MS çalışmaları kullanarak onların yapısal analiz sentez seçici fırsatlar.

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Kimya sayı: 140 peptidler sistein-zengin disülfür bağlantısı sentez koruma grubu strateji yapı analizi 2D NMR (nükleer manyetik rezonans) MS/MS (tandem kütle spektrometresi)
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