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Biochemistry

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JoVE Journal Biochemistry
Analyse af tylakoid membran proteinkomplekser af blå indfødte gelelektroforese
Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.
Journal (Biochemistry)
Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.
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Journal (Biochemistry)
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Analyse af tylakoid membran proteinkomplekser af blå indfødte gelelektroforese

Article DOI: 10.3791/58369-v • 08:12 min • September 28th, 2018
September 28th, 2018 •
1, 1, 1
1Molecular Plant Biology, Department of Biochemistry, University of Turku

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En protokol til udredning af plante tylakoid protein kompleks organisation og sammensætning med blå indfødte polyacrylamid gel elektroforese (BN-side) og 2D-SDS-PAGE er beskrevet. Protokollen er optimeret til Arabidopsis thaliana, , men kan bruges til andre plantearter med mindre ændringer.

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Biokemi sag 139 BN-side 2D gelelektroforese tylakoid proteinkomplekser nativt proteinkomplekser tylakoid membran photosystem
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