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Biochemistry

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JoVE Journal Biochemistry
Analyse der Thylakoidmembran Membran-Protein-komplexe durch blaue Native Gelelektrophorese
Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
Journal (Biochemistry)
Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
Journal (Biochemistry)
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
 
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Analyse der Thylakoidmembran Membran-Protein-komplexe durch blaue Native Gelelektrophorese

Article DOI: 10.3791/58369-v • 08:12 min • September 28th, 2018
September 28th, 2018 •
1, 1, 1
1Molecular Plant Biology, Department of Biochemistry, University of Turku

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Ein Protokoll für die Aufklärung der Pflanze Thylakoidmembran Protein komplexe Organisation und Zusammensetzung mit blauen native Polyacrylamid gel-Elektrophorese (BN-Seite) und 2D-SDS-PAGE beschrieben. Das Protokoll ist optimiert für Arabidopsis Thaliana, aber für andere Pflanzenarten mit geringfügigen Modifikationen eingesetzt werden.

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Biochemie Ausgabe 139 BN-Seite 2D Gelelektrophorese Thylakoidmembran Proteinkomplexe systemeigene Proteinkomplexen Thylakoidmembran Membran photosystem
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