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JoVE Journal Cancer Research
Phospho flowcytometri med fluorescerende celle Barcoding for enkelt celle signalisering analyse og biomarkør oppdagelsen
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Phospho flowcytometri med fluorescerende celle Barcoding for enkelt celle signalisering analyse og biomarkør oppdagelsen

Article DOI: 10.3791/58386-v • 08:38 min • October 4th, 2018
October 4th, 2018 •
1,2
1Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, University of Oslo, 2K. G. Jebsen Centre for B cell malignancies and K. G. Jebsen Centre for Cancer Immunotherapy, University of Oslo

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Her vises en protokoll for middels til høy gjennomstrømming analyse av protein fosforylering hendelser på cellenivå. Phospho-flowcytometri er en effektiv tilnærming til å karakterisere signalnettverk avvik, identifisere og validere biomarkers og vurdere pharmacodynamics.

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Kreftforskning problemet 140 biomarkør celle signalisering kronisk lymfatisk leukemi (CLL) fluorescerende celle Barcoding (FCB) Phospho flowcytometri Phospho-proteiner enkelt celle profilering
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