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Identifikasjon og histologiske karakteristikken Disseksjon av musen prostata Lobes for i Vitro 3D Spheroid kultur modeller
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Identifikasjon og histologiske karakteristikken Disseksjon av musen prostata Lobes for i Vitro 3D Spheroid kultur modeller

Article DOI: 10.3791/58397-v • 08:43 min • September 18th, 2018
September 18th, 2018 •
1,2, 3,4, 1, 1,2
1Department of Urology, SUNY Upstate Medical University, 2Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 3Laboratory of Comparative Pathology, Memorial Sloan-Kettering Cancer Center, 4Boulder BioPATH, Inc

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Genmodifiserte mus er nyttige modeller for å undersøke prostatakreft mekanismer. Her presenterer vi en protokoll for å identifisere og dissekere prostata lobes fra en mus urogenital system, skille dem basert på histology, og isolere og kultur primære prostata celler i vitro som spheroids for nedstrøms analyser.

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Kreftforskning problemet 139 musen prostata lobes prostata disseksjon prostata histology primære kultur 3D kultur musen prostata spheroids
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