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Isolasjon av voksen ryggmargen kjerner for massivt parallelle Single-kjernen RNA sekvensering
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Isolasjon av voksen ryggmargen kjerner for massivt parallelle Single-kjernen RNA sekvensering

Article DOI: 10.3791/58413-v • 06:38 min • October 12th, 2018
October 12th, 2018 •
1, 1, 2, 3,4, 3, 1
1Spinal Circuits and Plasticity Unit, National Institute of Neurological Disorders and Stroke, 2Bioinformatics Section, Information Technology Program, National Institute of Neurological Disorders and Stroke, 3Laboratory of Cochlear Development, National Institute on Deafness and Other Communication Disorders, 4Single Cell Analysis Facility, Frederick National Laboratory

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Her presenterer vi en protokoll for å raskt isolere høykvalitets kjerner fra friske eller frosne vev for nedstrøms massivt parallelle RNA sekvensering. Vi inkluderer vaskemiddel mekaniske og hypotonisk mekaniske vev avbrudd og celle lysis alternativer, begge kan brukes for isolering av kjerner.

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Nevrovitenskap problemet 140 kjerner RNA sekvensering snRNA-Seq massivt parallell ryggmarg sukrose gradering
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