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JoVE Journal Cancer Research
Overvågning Hippo signalering Pathway aktivitet ved hjælp af en Luciferase-baserede store Tumor Suppressor (LATS) Biosensor
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Overvågning Hippo signalering Pathway aktivitet ved hjælp af en Luciferase-baserede store Tumor Suppressor (LATS) Biosensor

Article DOI: 10.3791/58416-v • 07:16 min • September 13th, 2018
September 13th, 2018 •
*1, *1, 1, 1, 1
1Department of Pathology and Molecular Medicine, Queen's University
* These authors contributed equally

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Her præsenterer vi en luciferase-baserede biosensor for at kvantificere kinase aktiviteten af stor tumor suppressor (LATS)-en central kinase i Hippo signalering pathway. Denne biosensor har forskellige programmer i basic og Translationel forskning med henblik på at undersøge Hippo pathway lovgivere i in vitro og i vivo.

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Kræftforskning sag 139 LATS LATS biosensor Hippo pathway split luciferase assay cellekultur stof screening kinase
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