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JoVE Journal Genetics
Reverse transcriptie-lus-gemedieerde isotherme amplificatie (RT-LAMP) Assay voor Zika-Virus en huishouden genen in Urine, Serum en Mosquito monsters
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Reverse transcriptie-lus-gemedieerde isotherme amplificatie (RT-LAMP) Assay voor Zika-Virus en huishouden genen in Urine, Serum en Mosquito monsters

Article DOI: 10.3791/58436-v • 08:03 min • September 14th, 2018
September 14th, 2018 •
1, 2, 2, 1,3, 1,3
1Department of Urology, Beaumont Health System, 2Foundational Sciences, College of Medicine, Central Michigan University, 3Oakland University William Beaumont School of Medicine

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Dit protocol biedt een efficiënte en goedkope methode speurder Zika virus te bepalen van doelen in menselijke urine en serum monsters of muggen door omgekeerde transcriptie lus-gemedieerde isotherme amplificatie (RT-LAMP). Deze methode vereist geen RNA isolatie en kan worden gedaan binnen 30 min.

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Genetica kwestie 139 Zika Virus RT-LAMP urine serum Aedes point-of-care testing veld tools
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