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Journal / Medicine / Génération de Cultures de tissus pulmonaires…
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JoVE Journal Medicine
Génération de Cultures de tissus pulmonaires humains de 3D (3D-SFLC) pour la modélisation de la maladie
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Génération de Cultures de tissus pulmonaires humains de 3D (3D-SFLC) pour la modélisation de la maladie

Article DOI: 10.3791/58437-v • 05:47 min • February 12th, 2019
February 12th, 2019 •
1,2,3, 4,5,6, 4,5,6, 2,7, *1,2,3, *1,2,3,8
1Comprehensive Pneumology Center, Ludwig-Maximilians-Universität and Helmholtz Zentrum Munich, 2German Center of Lung Research (DZL), 3Translational Lung Research and CPC-M bioArchive, Helmholtz Zentrum München, Comprehensive Pneumology Center Munich DZL/CPC-M, 4Department of Experimental Medical Science, Lung Bioengineering and Regeneration, Lund University, 5Wallenberg Center for Molecular Medicine, Lund University, 6Stem Cell Centre, Lund University, 7Asklepios Fachkliniken Munich-Gauting, 8Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado
* These authors contributed equally

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Nous présentons ici un protocole pour la préparation de tranches de poumon de coupe de précision humain rempli d’agarose de tissus réséqués du patient qui sont aptes à produire des cultures de tissus pulmonaires 3D aux maladies pulmonaires humaines modèle dans études biologiques et biomédicales.

Tags

Médecine numéro 144 3D tissue culture culture ex-vivo des tissus tranches de coupe de précision poumon PCLS 3D-Olt modèles animaux de maladies modèles homme ex-vivo pneumopathie fibrose pulmonaire
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