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Journal / Medicine / Generation von menschlichen 3D Lungengewebe…
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JoVE Journal Medicine
Generation von menschlichen 3D Lungengewebe Kulturen (3D-LTCs) für Krankheit Modellierung
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Generation von menschlichen 3D Lungengewebe Kulturen (3D-LTCs) für Krankheit Modellierung

Article DOI: 10.3791/58437-v • 05:47 min • February 12th, 2019
February 12th, 2019 •
1,2,3, 4,5,6, 4,5,6, 2,7, *1,2,3, *1,2,3,8
1Comprehensive Pneumology Center, Ludwig-Maximilians-Universität and Helmholtz Zentrum Munich, 2German Center of Lung Research (DZL), 3Translational Lung Research and CPC-M bioArchive, Helmholtz Zentrum München, Comprehensive Pneumology Center Munich DZL/CPC-M, 4Department of Experimental Medical Science, Lung Bioengineering and Regeneration, Lund University, 5Wallenberg Center for Molecular Medicine, Lund University, 6Stem Cell Centre, Lund University, 7Asklepios Fachkliniken Munich-Gauting, 8Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado
* These authors contributed equally

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Hier präsentieren wir Ihnen ein Protokoll für die Vorbereitung der Agarose-gefüllte menschlichen präzise geschliffenen Lunge Scheiben aus resezierten Patienten Gewebe, die für die Erzeugung von 3D Lunge Gewebekulturen, Modell menschlichen Lungenerkrankungen in biologischen und biomedizinischen Studien eignen.

Tags

Medizin Ausgabe 144 3D Gewebekultur Ex-Vivo Gewebekultur Precision-Cut Lung Slices PCLS Lungenerkrankungen tierische Krankheitsmodelle menschliche Ex-Vivo-Modelle 3D-LTCs Lungenfibrose
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