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Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart
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Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart

Article DOI: 10.3791/58448-v • 11:45 min • January 7th, 2019
January 7th, 2019 •
1, 1,2
1Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, 2Department of Anesthesiology, University of Nebraska Medical Center

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Summary
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The overall goal of this article is to standardize the protocol for the isolation, characterization, and differentiation of cardiac stem cells (CSCs) from the adult mouse heart. Here, we describe a density gradient centrifugation method to isolate murine CSCs and elaborated methods for CSC culture, proliferation, and differentiation into cardiomyocytes.

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Isolation Characterization Differentiation Cardiac Stem Cells Adult Mouse Heart Protocol Cardiovascular Disease Regional Therapy Cardiac Stem Cell Therapy Heart Failure Cost-effective Small Period Of Time Materials Instruments Isolation Buffers Sterilized Condition Euthanized Mouse Ribcage Thoracic Cavity Heart Exposure Blood Removal One Milliliter Syringe Ice Cold PBS Surgical Scissors Tweezers Petri Dish Ham's Balanced Salt Solution
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