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Isolation, karakterisering og differentiering af Cardiac stamceller fra voksne mus hjerte
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Isolation, karakterisering og differentiering af Cardiac stamceller fra voksne mus hjerte

Article DOI: 10.3791/58448-v • 11:45 min • January 7th, 2019
January 7th, 2019 •
1, 1,2
1Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, 2Department of Anesthesiology, University of Nebraska Medical Center

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Det overordnede mål med denne artikel er at standardisere protokol for isolation, karakterisering og differentiering af cardiac stamceller (CSCs) fra voksne mus hjerte. Her, beskriver vi en tæthed gradient centrifugering metode til at isolere murine CSCs og omstændige metoder til CSC kultur, spredning og differentiering i cardiomyocytes.

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