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Isolatie, karakterisering en differentiatie van cardiale stamcellen van het hart volwassen muis
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Isolatie, karakterisering en differentiatie van cardiale stamcellen van het hart volwassen muis

Article DOI: 10.3791/58448-v • 11:45 min • January 7th, 2019
January 7th, 2019 •
1, 1,2
1Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, 2Department of Anesthesiology, University of Nebraska Medical Center

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Het algemene doel van dit artikel is op de standaardisering van het protocol voor de isolatie, karakterisering en differentiatie van cardiale stamcellen (CSCs) vanuit het hart van de volwassen muis. Hier beschrijven we een dichtheid kleurovergang centrifugeren methode om te isoleren lymfkliertest CSCs en uitgewerkte methoden voor CSC cultuur, proliferatie en differentiatie in cardiomyocytes.

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Biologie kwestie 143 Sca-1 OCT4 Nanog Ki-67 NKX2-5 GATA4 actinin troponine ik
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