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JoVE Journal Biochemistry
Brug af elektron paramagnetisk resonans i biologiske prøver ved omgivende temperatur og 77 K
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Brug af elektron paramagnetisk resonans i biologiske prøver ved omgivende temperatur og 77 K

Article DOI: 10.3791/58461-v • 06:45 min • January 11th, 2019
January 11th, 2019 •
1, 1, 2, 3, 1
1Cardiovascular Pulmonary Research Laboratories and Pediatric Critical Care Medicine, Department of Pediatrics, University of Colorado Anschutz Medical Campus, 2Bruker BioSpin Corp, 3Department of Medicine, Division of Clinical Pharmacology, Vanderbilt University Medical Center

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Elektron paramagnetisk resonans (EPR) spektroskopi er en entydig metode til at måle frie radikaler. Brugen af selektive spin sonder giver mulighed for påvisning af frie radikaler i forskellige cellulære rum. Vi præsenterer en praktisk, effektiv metode til at indsamle biologiske prøver, som letter behandling, lagring og overførsel af prøver for EPR målinger.

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Biokemi spørgsmålet 143 elektron paramagnetisk resonans spin sonder CMH CPH mito-TEMPO-H superoxid reaktive ilt arter nitroxide superoxiddismutase polytetrafluorethylen slanger
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