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Journal / Genetics / 利用南方印迹和聚合酶链反应鉴定小鼠胚胎干细胞的同源重组事件…
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JoVE Journal Genetics
利用南方印迹和聚合酶链反应鉴定小鼠胚胎干细胞的同源重组事件
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利用南方印迹和聚合酶链反应鉴定小鼠胚胎干细胞的同源重组事件

Article DOI: 10.3791/58467-v • 08:01 min • November 20th, 2018
November 20th, 2018 •
*1,2, *1, *3, 1, 1, 1, 4, 5, 3, 1
1Lab of Animal Models and Functional Genomics (LAMFG), The Key Laboratory of Animal Vaccine & Protein Engineering, College of Veterinary Medicine, Hunan Agricultural University (HUNAU), 2Department of Pathology, Georgetown University Medical School, 3College of Food Science and Technology, Hunan Agricultural University (HUNAU), 4Lab of Molecular Cardiology (LMC), National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH), 5Transgenic Core, National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH)
* These authors contributed equally

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在这里, 我们提出了一个详细的协议, 以确定同源重组事件发生在小鼠胚胎干细胞使用南方印迹和/或 pcr。利用传统的胚胎干细胞同源重组介导靶向技术生成的非肌肉肌球蛋白 ii 基因替代小鼠模型就是例证。

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遗传学 第141期 同源重组 基因靶向 南方印迹 pcr 小鼠 胚胎干细胞 探针 基因替代 敲除/敲入 myh9 基因 基因组 dna
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