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Journal / Genetics / Identifikasjon av homologe rekombinasjon…
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JoVE Journal Genetics
Identifikasjon av homologe rekombinasjon hendelser i mus embryonale stamceller Southern Blotting og polymerasekjedereaksjons
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Identifikasjon av homologe rekombinasjon hendelser i mus embryonale stamceller Southern Blotting og polymerasekjedereaksjons

Article DOI: 10.3791/58467-v • 08:01 min • November 20th, 2018
November 20th, 2018 •
*1,2, *1, *3, 1, 1, 1, 4, 5, 3, 1
1Lab of Animal Models and Functional Genomics (LAMFG), The Key Laboratory of Animal Vaccine & Protein Engineering, College of Veterinary Medicine, Hunan Agricultural University (HUNAU), 2Department of Pathology, Georgetown University Medical School, 3College of Food Science and Technology, Hunan Agricultural University (HUNAU), 4Lab of Molecular Cardiology (LMC), National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH), 5Transgenic Core, National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH)
* These authors contributed equally

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Her presenterer vi en detaljert protokoll for å identifisere homologe rekombinasjon hendelsene skjedde i mus embryonale stamceller bruker Southern blotting og/eller PCR. Denne metoden er eksemplifisert ved generering av nonmuscle myosin II genetisk erstatning musen modeller med tradisjonelle embryonale stamcelleforskningen-baserte homologe rekombinasjon-mediert teknologi.

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Genetikk problemet 141: homologe rekombinasjon gene målretting Southern blotting PCR mus embryonale stamceller sonde primer genetisk erstatning knockout/knock-inn Myh9 genet genomisk DNA
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