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Journal / Genetics / Identifiering av homolog rekombination händelser…
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Genetics

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JoVE Journal Genetics
Identifiering av homolog rekombination händelser i mus embryonala stamceller med södra Blotting och Polymerase Chain Reaction
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Identifiering av homolog rekombination händelser i mus embryonala stamceller med södra Blotting och Polymerase Chain Reaction

Article DOI: 10.3791/58467-v • 08:01 min • November 20th, 2018
November 20th, 2018 •
*1,2, *1, *3, 1, 1, 1, 4, 5, 3, 1
1Lab of Animal Models and Functional Genomics (LAMFG), The Key Laboratory of Animal Vaccine & Protein Engineering, College of Veterinary Medicine, Hunan Agricultural University (HUNAU), 2Department of Pathology, Georgetown University Medical School, 3College of Food Science and Technology, Hunan Agricultural University (HUNAU), 4Lab of Molecular Cardiology (LMC), National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH), 5Transgenic Core, National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH)
* These authors contributed equally

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Här presenterar vi ett detaljerat protokoll för att identifiera homolog rekombination händelser som inträffat i mus embryonala stamceller använder södra blotting eller PCR. Denna metod exemplifieras av generationen av nonmuscle myosin II genetisk utbyte musmodeller med traditionella embryonala stamceller-baserade homolog rekombination-medierad inriktningsteknik.

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Genetik fråga 141 homolog rekombination genmodifiering södra blotting PCR mus embryonala stamceller sond primer genetisk utbyte knockout/inpressning Myh9 gen genomisk DNA
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