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Journal / Engineering / Label-Free Imaging of Single Proteins Secreted…
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Engineering

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JoVE Journal Engineering
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Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

Article DOI: 10.3791/58486-v • 10:55 min • November 20th, 2018
November 20th, 2018 •
1, 1,2, 1,3, 4, 4, 1,3
1Max Planck Institute for the Science of Light (MPL), 2Area of Scientific Learning, Milligan College, 3Department of Physics, Friedrich Alexander University Erlangen-Nuremberg, 4Department of Internal Medicine 5, Hematology and Oncology, Friedrich Alexander University Erlangen-Nuremberg (FAU), University Hospital Erlangen

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Summary
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We present a protocol for the real-time optical detection of single unlabeled proteins as they are secreted from living cells. This is based on interferometric scattering (iSCAT) microscopy, which can be applied to a variety of different biological systems and configurations.

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Label-free Imaging Single Proteins Living Cells ISCAT Microscopy Detection And Spectroscopy Gold Nanoparticles Biological Nanoparticles Extinction Cross Section Sensitivity Scattering Background ISCAT Microscope Home Built Commercial Microscope Optical Techniques LUZ Cells Model System Secretory Proteins Molecular Level Stable Microscope
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