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Journal / Engineering / Label-fri Imaging af enkelt proteiner udskilles…
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Engineering

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JoVE Journal Engineering
Label-fri Imaging af enkelt proteiner udskilles fra levende celler via iSCAT mikroskopi
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Label-fri Imaging af enkelt proteiner udskilles fra levende celler via iSCAT mikroskopi

Article DOI: 10.3791/58486-v • 10:55 min • November 20th, 2018
November 20th, 2018 •
1, 1,2, 1,3, 4, 4, 1,3
1Max Planck Institute for the Science of Light (MPL), 2Area of Scientific Learning, Milligan College, 3Department of Physics, Friedrich Alexander University Erlangen-Nuremberg, 4Department of Internal Medicine 5, Hematology and Oncology, Friedrich Alexander University Erlangen-Nuremberg (FAU), University Hospital Erlangen

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Vi præsenterer en protokol til real-time optisk påvisning af enkelt umærket proteiner som de udskilles fra levende celler. Dette er baseret på interferometriske spredning (iSCAT) mikroskopi, som kan anvendes til en række forskellige biologiske systemer og konfigurationer.

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Engineering sag 141 iSCAT label-fri single-protein cellulære sekretion billedbehandling spredning dynamics real-time
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