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Journal / Neuroscience / Cryosectioning simultanée de plusieurs cerveaux…
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JoVE Journal Neuroscience
Cryosectioning simultanée de plusieurs cerveaux de rongeurs
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Cryosectioning simultanée de plusieurs cerveaux de rongeurs

Article DOI: 10.3791/58513-v • 06:37 min • September 18th, 2018
September 18th, 2018 •
1,2,3, 1, 5, 1,3,4, 1,3,4
1Department of Child Health, University of Arizona College of Medicine, 2Department of Biological Sciences, University of Bath, 3BARROW Neurological Institute at Phoenix Children's Hospital, 4Phoenix Veteran Affairs Healthcare System, 5Department of Basic Medical Sciences, University of Arizona College of Medicine

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Nous présentons ici un protocole visant à geler et l’article de tissus cérébraux de plusieurs animaux comme une alternative de gagner du temps aux cerveaux unique de transformation. Cela réduit la variabilité coloration en immunohistochimie et réduit d’imagerie et de temps cryosectioning.

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Neurosciences numéro 139 Neuroscience histologie immunohistochimie rongeur cryoprotection cryosectioning cryostat neuropathologie
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