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Bioengineering

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JoVE Journal Bioengineering
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A Zebrafish Embryo Model for In Vivo Visualization and Intravital Analysis of Biomaterial-associated Staphylococcus aureus Infection

Article DOI: 10.3791/58523-v • 10:04 min • January 7th, 2019
January 7th, 2019 •
1,2, 1, 1, 2,3, 4, 1
1Department of Medical Microbiology, Amsterdam UMC, 2Technical Medical Center, Department of Biomaterials Science and Technology, University of Twente, 3Department of Biomedical Engineering, W.J. Kolff Institute, University Medical Center Groningen, University of Groningen, 4Institute of Biology, Leiden University

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The present study describes a zebrafish embryo model for in vivo visualization and intravital analysis of biomaterial-associated infection over time based on fluorescence microscopy. This model is a promising system complementing mammalian animal models such as mouse models for studying biomaterial-associated infections in vivo.

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Zebrafish Embryo Model In Vivo Visualization Intravital Analysis Biomaterial-associated Staphylococcus Aureus Infection Biomaterials Susceptibility To Infection Progression Of Infections Immune Cell Behavior Animal Model Biomaterial Microspheres Bacteria-only Suspension Fluorescent Staph Aureus MCherry Strain Tryptic Soy Agriculture Plates Chloramphenicol Tryptic Soy Broth Mid-logarithmic Growth Phase Optical Density Measurement
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