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Journal / Biochemistry / Affiniteit zuivering van Chloroplast Translocon…
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Biochemistry

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JoVE Journal Biochemistry
Affiniteit zuivering van Chloroplast Translocon eiwitcomplexen met behulp van de Tag van de TAP
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Affiniteit zuivering van Chloroplast Translocon eiwitcomplexen met behulp van de Tag van de TAP

Article DOI: 10.3791/58532-v • 07:01 min • November 1st, 2018
November 1st, 2018 •
1, 1, 2, 1
1Laboratory of Plant Physiology, University of Neuchatel, 2Institut fur Biochemie und Biotechnologie, Martin-Luther-Universitat Halle-Wittenberg

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Hier presenteren we een bewezen en geteste protocol voor de zuivering van chloroplast importeren eiwitcomplexen (TOC-TIC complex) met behulp van de TAP-tag. Het protocol van de verwantschap-isolatie one-step potentieel kan worden toegepast op elke proteïne en worden gebruikt om nieuwe interactie partners door massaspectrometrie.

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Biochemie kwestie 141 Chloroplast TOC-TIC Complex TAP tag TOC159 IgG bindend TEV protease affiniteit zuivering.
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