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Journal / Biochemistry / Meten van interacties van bolvormige en…
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Biochemistry

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JoVE Journal Biochemistry
Meten van interacties van bolvormige en filamenteuze eiwitten door nucleaire magnetische resonantie spectroscopie (NMR) en Microscale Thermophoresis (MST)
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Meten van interacties van bolvormige en filamenteuze eiwitten door nucleaire magnetische resonantie spectroscopie (NMR) en Microscale Thermophoresis (MST)

Article DOI: 10.3791/58537-v • 10:28 min • November 2nd, 2018
November 2nd, 2018 •
1, 1, 1, 1
1Department of Biochemistry, University of Alberta

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Hier presenteren we een protocol voor de productie en zuivering van eiwitten die zijn gemarkeerd met stabiele isotopen, en verdere karakterisering van eiwit-eiwitinteractie met behulp nucleaire magnetische resonantie (NMR) spectroscopie en MicroScale Thermophoresis (MST) experimenten.

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Biochemie kwestie 141 cel adhesiondesmosome desmoplakin draadvormige proteïne intermediaire filamenten microscale thermophoresis nucleaire magnetische resonantie spectroscopie eiwit interactie vimentin
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