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Biochemistry

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JoVE Journal Biochemistry
Strukturelle studier av makromolekyler i løsning med liten vinkel X-Ray spredning
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Strukturelle studier av makromolekyler i løsning med liten vinkel X-Ray spredning

Article DOI: 10.3791/58538-v • 07:19 min • November 5th, 2018
November 5th, 2018 •
1, 2, 2,4, 1,3,4
1Alberta RNA Research and Training Institute, Department of Chemistry and Biochemistry, University of Lethbridge, 2Department of Biochemistry, University of Alberta, 3Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary, 4DiscoveryLab, Faculty of Medicine & Dentistry, University of Alberta

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Her presenterer vi hvor liten vinkel X-Ray spredning (SAXS) kan brukes til å få informasjon om lav oppløsning konvolutter representerer macromolecular strukturer. Når den brukes sammen med høy oppløsning strukturelle teknikker som Røntgenkrystallografi og kjernefysiske magnetisk resonans, kan SAXS gi detaljert innblikk i replikasjonen proteiner og macromolecular komplekser i-løsning.

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Biokjemi problemet 141 ekstracellulær matrix proteiner hybrid løsning struktur laminin trafikk protein nidogen protein samhandling proteinstruktur strukturell domene liten vinkel X-ray spredning tredimensjonal modell
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