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Journal / Biochemistry / Structurele Studies van macromoleculen in…
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Biochemistry

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JoVE Journal Biochemistry
Structurele Studies van macromoleculen in oplossing met behulp van kleine Angle X-Ray Scattering
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Structurele Studies van macromoleculen in oplossing met behulp van kleine Angle X-Ray Scattering

Article DOI: 10.3791/58538-v • 07:19 min • November 5th, 2018
November 5th, 2018 •
1, 2, 2,4, 1,3,4
1Alberta RNA Research and Training Institute, Department of Chemistry and Biochemistry, University of Lethbridge, 2Department of Biochemistry, University of Alberta, 3Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary, 4DiscoveryLab, Faculty of Medicine & Dentistry, University of Alberta

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Hier presenteren we hoe kleine Angle X-Ray Scattering (SAXS) kan worden gebruikt om informatie op lage resolutie enveloppen die vertegenwoordigen de macromoleculaire structuur te verkrijgen. Wanneer gebruikt in combinatie met hoge resolutie structurele technieken zoals röntgendiffractie en nucleaire magnetische resonantie, bieden SAXS gedetailleerde inzichten in multidomain eiwitten en macromoleculaire complexen in-oplossing.

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Biochemie kwestie 141 extracellulaire matrix eiwitten hybride oplossing structuur laminin met meerdere domeinen eiwit nidogen eiwit interactie eiwitstructuur structurele domein kleine hoek X-ray verstrooiing driedimensionaal model
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