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Journal / Biochemistry / 肾小球的分离及肾小球细胞表面蛋白的活体标记…
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Biochemistry

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JoVE Journal Biochemistry
肾小球的分离及肾小球细胞表面蛋白的活体标记
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肾小球的分离及肾小球细胞表面蛋白的活体标记

Article DOI: 10.3791/58542-v • 09:12 min • January 18th, 2019
January 18th, 2019 •
1, 1, 1, 2, 1, 1, 1, 1, 1, 1
1Department of Nephrology, Medical Faculty, Heinrich-Heine-University, 2Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf

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在这里, 我们提出了一个协议的小鼠体内标记肾小球细胞表面蛋白与生物素.该协议包含有关如何灌注小鼠肾脏, 分离肾小球, 并执行内源性免疫沉淀的感兴趣的蛋白质的信息。

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生物化学 第143期 体内生物素化 细胞表面蛋白标记 肾小球 肾小球隔离 磁珠 内吞 体内蛋白质贩运
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