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JoVE Journal
Biochemistry

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Isolering af Glomeruli og In Vivo mærkning af glomerulær celle overflade proteiner
 

Isolering af Glomeruli og In Vivo mærkning af glomerulær celle overflade proteiner

Article doi: 10.3791/58542
January 18th, 2019

Summary January 18th, 2019

Please note that all translations are automatically generated.

Click here for the English version.

Her præsenterer vi en protokol for murine i vivo mærkning af glomerulær celle overflade proteiner med biotin. Denne protokol indeholder oplysninger om, hvordan du perfuse musen nyrer, isolere glomeruli og udføre endogene immunoprecipitation af protein af interesse.

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