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Journal / Genetics / Pianta di crescita e trasformazione di Floral-dip …
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JoVE Journal Genetics
Pianta di crescita e trasformazione di Floral-dip mediata da Agrobacterium della Extremophyte Schrenkiella parvula
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Pianta di crescita e trasformazione di Floral-dip mediata da Agrobacterium della Extremophyte Schrenkiella parvula

Article DOI: 10.3791/58544-v • 06:32 min • January 7th, 2019
January 7th, 2019 •
1, 1, 1, 1, 1
1Department of Biological Sciences, Louisiana State University

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Trasformazione mediata da Agrobacterium utilizzando un metodo floreale-dip può essere impiegato con successo per creare linee transgeniche stabile del modello extremophyte Schrenkiella parvula. Vi presentiamo un protocollo modificato da quello per Arabidopsis thaliana, considerando le abitudini differenti di sviluppo e caratteristiche fisiologiche della extremophyte.

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Genetica problema 143 Extremophyte Schrenkiella parvula Thellungiella parvula Eutrema parvulum floreale-dip impianto di selezione di trasformazione Agrobacterium dei transformants
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