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Journal / Genetics / 文字列アセンブリ gRNA のカスタマイズ可能なプロトコル (STAgR)…
JoVE Journal
Genetics

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JoVE Journal Genetics
文字列アセンブリ gRNA のカスタマイズ可能なプロトコル (STAgR) のクローニング
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文字列アセンブリ gRNA のカスタマイズ可能なプロトコル (STAgR) のクローニング

Article DOI: 10.3791/58556-v • 10:00 min • December 26th, 2018
December 26th, 2018 •
1,2, 1,2, 3, 3, 2,4, 1,2
1MCN Junior Research Group, Munich Center for Neurosciences, Ludwig Maximilian Universitat, BioMedical Center, 2Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health, 3Institute of Developmental Genetics, Helmholtz Zentrum, German Research Center for Environmental Health, 4Physiological Genomics, Ludwig Maximilian Universitat, BioMedical Center

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文字列アセンブリ gRNA (STAgR) のクローニングを紹介ここでは、簡単に CRISPR/Cas9 のアプローチの gRNA のベクトルを多重化する方法。STAgR は、シンプルで効率的かつカスタマイズ可能な gRNA 多重になります。

Tags

遺伝学の問題 142、CRISPR、Cas9、dCas9、gRNA のクローニング、gRNA 多重、ゲノム編集、トランスクリプトームの編集
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