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Journal / Genetics / 사용자 정의 프로토콜 문자열 어셈블리 gRNA에 대 한 복제…
JoVE Journal
Genetics

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JoVE Journal Genetics
사용자 정의 프로토콜 문자열 어셈블리 gRNA에 대 한 복제 (STAgR)
Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes
Journal (Genetics)
Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes
Purification of Low-abundant Cells in the Drosophila Visual System
Journal (Genetics)
Purification of Low-abundant Cells in the Drosophila Visual System
 
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사용자 정의 프로토콜 문자열 어셈블리 gRNA에 대 한 복제 (STAgR)

Article DOI: 10.3791/58556-v • 10:00 min • December 26th, 2018
December 26th, 2018 •
1,2, 1,2, 3, 3, 2,4, 1,2
1MCN Junior Research Group, Munich Center for Neurosciences, Ludwig Maximilian Universitat, BioMedical Center, 2Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health, 3Institute of Developmental Genetics, Helmholtz Zentrum, German Research Center for Environmental Health, 4Physiological Genomics, Ludwig Maximilian Universitat, BioMedical Center

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여기, 선물이 문자열 어셈블리 gRNA 복제 (STAgR), CRISPR/Cas9 접근에 쉽게 gRNA 벡터를 다중화 하는 방법. STAgR은 gRNA 멀티플렉싱 간단 하 고 효율적인 사용자 정의 합니다.

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유전학 문제점 142 CRISPR Cas9 dCas9 gRNA 복제 gRNA 멀티플렉싱 편집 게놈 transcriptome 편집
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