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Journal / Biology / Ensayo de absorción de colesterol LDL utilizando…
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Ensayo de absorción de colesterol LDL utilizando celular directo imágenes análisis con células control de salud
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Ensayo de absorción de colesterol LDL utilizando celular directo imágenes análisis con células control de salud

Article DOI: 10.3791/58564-v • 08:45 min • November 17th, 2018
November 17th, 2018 •
*1,2, *1,3, 4, 4,5, 6, 1,2,7,8
1Interdisciplinary Stem Cell Institute, University of Miami Leonard M. Miller School of Medicine, 2Department of Medicine, Division of Cardiology, University of Miami Leonard M. Miller School of Medicine, 3Department of Molecular and Cellular Pharmacology, University of Miami Leonard M. Miller School of Medicine, 4Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami Leonard M. Miller School of Medicine, 5John P. Hussman Institute for Human Genomics, University of Miami Leonard M. Miller School of Medicine, 6Department of Medicine, Division of Endocrinology, Metabolism and Endocrinology and the Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, 7Vascular Biology Institute, University of Miami Leonard M. Miller School of Medicine, 8Peggy and Harold Katz Family Drug Discovery Center, University of Miami Leonard M. Miller School of Medicine
* These authors contributed equally

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Este protocolo proporciona un enfoque eficiente para medir la absorción de colesterol LDL con tasas de flujo en tiempo real usando un sistema en diversos tipos celulares de imagen vivo de la célula. Esta técnica proporciona una plataforma para la actividad farmacológica de compuestos que afectan flujo de LDL mientras para morfología de la célula y por lo tanto potencial citotoxicidad de la pantalla.

Tags

Biología número 141 lipoproteína de baja densidad LDLR afluencia de LDL LDL captación Dynasore simvastatina PCSK9 recombinante Dinamina lípidos colesterol arteriosclerosis.
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