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Journal / Biology / Test d’absorption LDL cholestérol analyse avec…
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Biology

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JoVE Journal Biology
Test d’absorption LDL cholestérol analyse avec cellule d’imagerie de cellules vivantes surveillance sanitaire
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Test d’absorption LDL cholestérol analyse avec cellule d’imagerie de cellules vivantes surveillance sanitaire

Article DOI: 10.3791/58564-v • 08:45 min • November 17th, 2018
November 17th, 2018 •
*1,2, *1,3, 4, 4,5, 6, 1,2,7,8
1Interdisciplinary Stem Cell Institute, University of Miami Leonard M. Miller School of Medicine, 2Department of Medicine, Division of Cardiology, University of Miami Leonard M. Miller School of Medicine, 3Department of Molecular and Cellular Pharmacology, University of Miami Leonard M. Miller School of Medicine, 4Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami Leonard M. Miller School of Medicine, 5John P. Hussman Institute for Human Genomics, University of Miami Leonard M. Miller School of Medicine, 6Department of Medicine, Division of Endocrinology, Metabolism and Endocrinology and the Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, 7Vascular Biology Institute, University of Miami Leonard M. Miller School of Medicine, 8Peggy and Harold Katz Family Drug Discovery Center, University of Miami Leonard M. Miller School of Medicine
* These authors contributed equally

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Ce protocole prévoit une approche efficace pour mesurer l’absorption de cholestérol LDL avec des taux de flux en temps réel à l’aide d’un système dans divers types de cellules d’imagerie de cellules vivantes. Cette technique fournit une plate-forme pour l’activité pharmacologique de composés affectant les afflux de LDL tout en surveillant pour la morphologie cellulaire et cytotoxicité donc potentielle de l’écran.

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Biologie numéro 141 lipoprotéines de faible densité LDLR afflux de LDL captation des LDL Dynasore simvastatine recombinant PCSK9 dynamine lipides cholestérol athérosclérose.
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