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JoVE Journal Neuroscience
Direct Injection intrathécale de Recombinant Adeno-associated virus chez la souris adulte
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Direct Injection intrathécale de Recombinant Adeno-associated virus chez la souris adulte

Article DOI: 10.3791/58565-v • 08:17 min • February 15th, 2019
February 15th, 2019 •
*1, *2, 1, 3, 1,4
1Department of Neurology, Second Hospital of Hebei Medical University, 2No.2 Middle School of Shijiazhuang, 3Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 4Key Laboratory of Hebei Neurology
* These authors contributed equally

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Nous présentons une technique d’injection intrathécale directe à l’aide de chlorhydrate de lidocaïne 1 % dans une solution virale pour assurer une prestation efficace virus adeno-associé à de petits animaux et de mettre en place un système de notation pour prédire l’efficacité de la transduction du central système nerveux selon le degré de faiblesse transitoire induit par la lidocaïne.

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Neurosciences numéro 144 intrathécale AAV transduction de gène système nerveux central du chlorhydrate de lidocaïne EGFP
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