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JoVE Journal Neuroscience
Diretto iniezione intratecale di ricombinanti Virus Adeno-associato in topi adulti
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Diretto iniezione intratecale di ricombinanti Virus Adeno-associato in topi adulti

Article DOI: 10.3791/58565-v • 08:17 min • February 15th, 2019
February 15th, 2019 •
*1, *2, 1, 3, 1,4
1Department of Neurology, Second Hospital of Hebei Medical University, 2No.2 Middle School of Shijiazhuang, 3Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 4Key Laboratory of Hebei Neurology
* These authors contributed equally

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Qui presentiamo una tecnica di iniezione intratecale diretto utilizzo 1% lidocaina cloridrato in una soluzione virale per garantire una consegna efficiente virus adeno-associato ai piccoli animali e stabilire un sistema di scoring per predire l'efficienza di trasduzione in zona centrale sistema nervoso secondo il grado della debolezza transitoria indotta da lidocaina.

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Neuroscienze problema 144 intratecale AAV trasduzione del gene sistema nervoso centrale lidocaina cloridrato EGFP
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