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JoVE Journal Neuroscience
Direkte Intratekal injektion af rekombinant Adeno-associeret virus i voksen mus
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Direkte Intratekal injektion af rekombinant Adeno-associeret virus i voksen mus

Article DOI: 10.3791/58565-v • 08:17 min • February 15th, 2019
February 15th, 2019 •
*1, *2, 1, 3, 1,4
1Department of Neurology, Second Hospital of Hebei Medical University, 2No.2 Middle School of Shijiazhuang, 3Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 4Key Laboratory of Hebei Neurology
* These authors contributed equally

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Her præsenterer vi en direkte Intratekal injektionsteknik bruger 1% lidocain hydrochlorid i en viral løsning at sikre effektiv adeno-associeret virus levering til små dyr og indføre et pointsystem for at forudsige transduktion effektivitet i centralt nervesystemet forhold til graden af forbigående svaghed induceret af lidocain.

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Neurovidenskab spørgsmålet 144 Intratekal AAV gen transduktion centralnervesystemet lidocain hydrochlorid EGFP
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