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JoVE Journal Neuroscience
Direkte intratekal injeksjon av rekombinant Adeno-assosiert virus i voksen mus
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Direkte intratekal injeksjon av rekombinant Adeno-assosiert virus i voksen mus

Article DOI: 10.3791/58565-v • 08:17 min • February 15th, 2019
February 15th, 2019 •
*1, *2, 1, 3, 1,4
1Department of Neurology, Second Hospital of Hebei Medical University, 2No.2 Middle School of Shijiazhuang, 3Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 4Key Laboratory of Hebei Neurology
* These authors contributed equally

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Her presenterer vi en direkte intratekal injeksjon teknikken bruker 1% lidokain hydroklorid i en viral løsning å sikre effektiv adeno-assosiert virus levering til små dyr og etablere et poengsystem for å forutsi signaltransduksjon effektivitet i central nervesystemet etter forbigående svakhet av lidocaine.

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Nevrovitenskap problemet 144 intratekal AAV gene signaltransduksjon sentralnervesystemet lidokain hydroklorid EGFP
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