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JoVE Journal
Immunology and Infection

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CRISPR-Cas9-baseret genom Engineering til at generere Jurkat Reporter modeller for HIV-1 infektion med udvalgte Proviral Integration websteder
 
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CRISPR-Cas9-baseret genom Engineering til at generere Jurkat Reporter modeller for HIV-1 infektion med udvalgte Proviral Integration websteder

Article doi: 10.3791/58572
November 14th, 2018

Summary November 14th, 2018

Please note that all translations are automatically generated.

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Vi præsenterer en genom engineering arbejdsgang for generation af nye in vitro-modeller for HIV-1 infektion, at sammenfatte proviral integration på udvalgte genomisk lokaliteter. Målretning af HIV-Afledte reportere lettes ved CRISPR-Cas9-medieret, lokationsspecifikke genom manipulation. Der findes detaljerede protokoller for encellede klon generation, screening og korrekte målretning verifikation.

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