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Journal / Immunology and Infection / CRISPR-Cas9-baseret genom Engineering til at…
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Immunology and Infection

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JoVE Journal Immunology and Infection
CRISPR-Cas9-baseret genom Engineering til at generere Jurkat Reporter modeller for HIV-1 infektion med udvalgte Proviral Integration websteder
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CRISPR-Cas9-baseret genom Engineering til at generere Jurkat Reporter modeller for HIV-1 infektion med udvalgte Proviral Integration websteder

Article DOI: 10.3791/58572-v • 14:27 min • November 14th, 2018
November 14th, 2018 •
*1,2, *1, 1,3, 1,2,3
1Heinrich Pette Institute, Leibniz Institute for Experimental Virology, 2Department of Anesthesiology, University Medical Center Hamburg-Eppendorf, 3German Center for Infection Research (DZIF)
* These authors contributed equally

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Vi præsenterer en genom engineering arbejdsgang for generation af nye in vitro-modeller for HIV-1 infektion, at sammenfatte proviral integration på udvalgte genomisk lokaliteter. Målretning af HIV-Afledte reportere lettes ved CRISPR-Cas9-medieret, lokationsspecifikke genom manipulation. Der findes detaljerede protokoller for encellede klon generation, screening og korrekte målretning verifikation.

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Immunologi og infektion sag 141 HIV HIV latenstid HIV integration websteder celle kultur model genom engineering CRISPR-Cas9
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