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Journal / Immunology and Infection / CRISPR-Cas9-baserte genomet Engineering generere…
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Immunology and Infection

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JoVE Journal Immunology and Infection
CRISPR-Cas9-baserte genomet Engineering generere Jurkat Reporter modeller for HIV-1 smitte med valgte Proviral integrasjon nettsteder
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CRISPR-Cas9-baserte genomet Engineering generere Jurkat Reporter modeller for HIV-1 smitte med valgte Proviral integrasjon nettsteder

Article DOI: 10.3791/58572-v • 14:27 min • November 14th, 2018
November 14th, 2018 •
*1,2, *1, 1,3, 1,2,3
1Heinrich Pette Institute, Leibniz Institute for Experimental Virology, 2Department of Anesthesiology, University Medical Center Hamburg-Eppendorf, 3German Center for Infection Research (DZIF)
* These authors contributed equally

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Vi presenterer en genomet engineering arbeidsflyt for generering av nye i vitro modeller for HIV-1 smitte som recapitulate proviral integrering på utvalgte genomisk områder. Målretting av HIV-avledet journalister er tilrettelagt av CRISPR-Cas9-mediert, områdespesifikke genomet manipulasjon. Detaljert protokoller for encellede klone generasjon, screening og riktig målretting verifisering tilbys.

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Ventetid for immunologi og infeksjon problemet 141 HIV HIV HIV integrering nettsteder celle kultur modell genomet engineering CRISPR-Cas9
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