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JoVE Journal Biochemistry
Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy
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Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy

Article DOI: 10.3791/58580-v • 09:36 min • October 19th, 2018
October 19th, 2018 •
*1,2, *1,2, 1,3, 1,2,3,4,5
1College of Chemistry and Molecular Engineering, Peking University, 2Beijing National Laboratory for Molecular Sciences, Peking University, 3Peking-Tsinghua Center for Life Sciences, Peking University, 4Synthetic and Functional Biomolecules Center, Peking University, 5Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University
* These authors contributed equally

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Summary
Automatic Translation

A detailed protocol for applying the click chemistry-assisted RNA-interactome capture (CARIC) strategy to identify proteins binding to both coding and noncoding RNAs is presented.

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Click Chemistry-assisted RNA-interactome Capture (CARIC) RNA-binding Proteins Post-transcriptional Gene Regulation MRNA Non-coding RNAs HeLa Cells Culture Medium EU Four SU In Vivo Cross-linking UV Cross-linker
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