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Journal / Biochemistry / Captura e identificação de proteínas de ligação…
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Biochemistry

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JoVE Journal Biochemistry
Captura e identificação de proteínas de ligação de RNA usando clique química assistida RNA-interactome capturam estratégia (CARIC)
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Captura e identificação de proteínas de ligação de RNA usando clique química assistida RNA-interactome capturam estratégia (CARIC)

Article DOI: 10.3791/58580-v • 09:36 min • October 19th, 2018
October 19th, 2018 •
*1,2, *1,2, 1,3, 1,2,3,4,5
1College of Chemistry and Molecular Engineering, Peking University, 2Beijing National Laboratory for Molecular Sciences, Peking University, 3Peking-Tsinghua Center for Life Sciences, Peking University, 4Synthetic and Functional Biomolecules Center, Peking University, 5Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University
* These authors contributed equally

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Um protocolo detalhado para a aplicação a clique química assistida RNA-interactome captura (CARIC) estratégia para identificar proteínas obrigatório para ambos os de codificação e não-codificante RNAs é apresentado.

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Bioquímica questão 140 RNA interações RNA-proteína proteômica opaco química RNA não-codificante
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