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Journal / Biochemistry / Capture et Identification des protéines de…
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Biochemistry

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JoVE Journal Biochemistry
Capture et Identification des protéines de liaison à l’ARN à l’aide de Click chimie assistée par ARN-interactome Capture stratégie (CARIC)
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
Journal (Biochemistry)
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
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Capture et Identification des protéines de liaison à l’ARN à l’aide de Click chimie assistée par ARN-interactome Capture stratégie (CARIC)

Article DOI: 10.3791/58580-v • 09:36 min • October 19th, 2018
October 19th, 2018 •
*1,2, *1,2, 1,3, 1,2,3,4,5
1College of Chemistry and Molecular Engineering, Peking University, 2Beijing National Laboratory for Molecular Sciences, Peking University, 3Peking-Tsinghua Center for Life Sciences, Peking University, 4Synthetic and Functional Biomolecules Center, Peking University, 5Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University
* These authors contributed equally

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Un protocole détaillé pour l’application de la clic chimie assistée par RNA-interactome capture (CARIC) stratégie pour identifier les protéines liant les deux codage et non codantes RNAs est présenté.

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Biochimie numéro 140 ARN interactions ARN-protéine protéomique bioorthogonal chimie non codante RNA
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