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Biochemistry

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JoVE Journal Biochemistry
Erfassung und Identifizierung von RNA-bindende Proteine mit Click-Chemie-gestützte RNA-Interaktom erfassen (CARIC) Strategie
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Erfassung und Identifizierung von RNA-bindende Proteine mit Click-Chemie-gestützte RNA-Interaktom erfassen (CARIC) Strategie

Article DOI: 10.3791/58580-v • 09:36 min • October 19th, 2018
October 19th, 2018 •
*1,2, *1,2, 1,3, 1,2,3,4,5
1College of Chemistry and Molecular Engineering, Peking University, 2Beijing National Laboratory for Molecular Sciences, Peking University, 3Peking-Tsinghua Center for Life Sciences, Peking University, 4Synthetic and Functional Biomolecules Center, Peking University, 5Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University
* These authors contributed equally

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Ein detailliertes Protokoll zur Anwendung der Klick Chemie-gestützte RNA-Interaktom erfassen (CARIC) Strategie um Proteine binden beide Codierung und Forensisches identifizieren RNAs wird vorgestellt.

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Biochemie Ausgabe 140 RNS RNS-Protein-Interaktionen Proteomik Bioorthogonal Chemie nichtcodierender RNA
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