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Biochemistry

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JoVE Journal Biochemistry
Fangst og identifikasjon av RNA-bindende proteiner ved hjelp av Klikk kjemi-assistert RNA-interactome Capture (CARIC) strategi
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Fangst og identifikasjon av RNA-bindende proteiner ved hjelp av Klikk kjemi-assistert RNA-interactome Capture (CARIC) strategi

Article DOI: 10.3791/58580-v • 09:36 min • October 19th, 2018
October 19th, 2018 •
*1,2, *1,2, 1,3, 1,2,3,4,5
1College of Chemistry and Molecular Engineering, Peking University, 2Beijing National Laboratory for Molecular Sciences, Peking University, 3Peking-Tsinghua Center for Life Sciences, Peking University, 4Synthetic and Functional Biomolecules Center, Peking University, 5Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University
* These authors contributed equally

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En detaljert protokoll for å bruke Klikk kjemi-assistert RNA-interactome capture (CARIC) strategi for å identifisere proteiner bindende til både koding og noncoding RNAs presenteres.

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Biokjemi problemet 140 RNA RNA-protein interaksjoner Proteomikk bioorthogonal kjemi noncoding RNA
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