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En fluorescens fluktuation spektroskopi haltbestämning av Protein-Protein interaktioner på Cell-Cell kontakter
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En fluorescens fluktuation spektroskopi haltbestämning av Protein-Protein interaktioner på Cell-Cell kontakter

Article DOI: 10.3791/58582-v • 08:43 min • December 1st, 2018
December 1st, 2018 •
1, 1
1Institute for Biochemistry and Biology, Cell Membrane Biophysics Group, University of Potsdam

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Det här protokollet beskriver ett fluorescens fluktuation spektroskopi-baserat tillvägagångssätt för att undersöka interaktioner mellan proteiner medla cell-cell interaktioner, dvs proteiner lokaliserade i cell korsningar, direkt i levande celler. Vi tillhandahåller detaljerade riktlinjer på instrumentkalibrering, datainsamling och analys, inklusive korrigeringar till möjlig artefakt källor.

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Biokemi fråga 142 Protein-protein interaktioner cell-cell interaktioner cell-cell adhesion fluorescens fluktuation spektroskopi fluorescens korrelation spektroskopi nummer och ljusstyrka N & B
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