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En fluorescens svingninger spektroskopi analysen av Protein-Protein interaksjoner i celle-celle kontakter
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En fluorescens svingninger spektroskopi analysen av Protein-Protein interaksjoner i celle-celle kontakter

Article DOI: 10.3791/58582-v • 08:43 min • December 1st, 2018
December 1st, 2018 •
1, 1
1Institute for Biochemistry and Biology, Cell Membrane Biophysics Group, University of Potsdam

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Denne protokollen beskriver en fluorescens svingninger spektroskopi tilnærming for å undersøke interaksjon mellom proteiner formidling celle-celle interaksjoner, dvs proteiner lokalisert i cellen veikryss, direkte i levende celler. Vi gir detaljerte veiledninger på instrumentet kalibrering, datainnsamling og analyse, inkludert rettelser til mulig gjenstanden kilder.

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Biokjemi problemet 142 Protein-protein interaksjoner celle-celle interaksjoner celle-celle adhesjon fluorescens svingninger spektroskopi fluorescens korrelasjon spektroskopi nummer og lysstyrke N & B
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