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Journal / Biochemistry / Nasıl Photoactivated floresan proteinler toplu…
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Biochemistry

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JoVE Journal Biochemistry
Nasıl Photoactivated floresan proteinler toplu olarak ve canlı hücrelerdeki kısmını ölçmek için
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Nasıl Photoactivated floresan proteinler toplu olarak ve canlı hücrelerdeki kısmını ölçmek için

Article DOI: 10.3791/58588-v • 11:03 min • January 7th, 2019
January 7th, 2019 •
1, 1
1Gynecologic Oncology Division, Stanford University School of Medicine

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Burada, genetik olarak eşleşmiş hayalice ayrı photoactivatable ve floresan proteinler içerir bir iletişim kuralı mevcut. Bu floresan protein chimeras miktar photoactivated Yani, floresan, olmak olduğunu PA-FP fraksiyonunun photoactivation verimliliği izin verir. Protokol photoactivation farklı modları farklı photoactivation verimliliği verim ortaya koymaktadır.

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Biyokimya sayı 143 Photoactivatable floresan proteinler photoactivation verimlilik ratiometric ensemble Yoğunluk merkezli çalışmalar photoconvertible floresan proteinler GFP mCherry
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